고형화제를 이용한 세균 고정, 약물 확산, 세균 단일 세포 추적과 이를 기반으로 한 빠른 약물 검사 시스템

Rapid antibiotic susceptibility test (rast) system based on bacterial fixation, antibiotic diffusion and single cell growth tracking using gelling agents

Abstract

PURPOSE: A test method using fixation of a biological substance and diffusion of a physiologically active substance is provided to largely reduce the amount of a drug and cells for a drug test, and to quickly trace single cell growth. CONSTITUTION: A physiological activity test method is performed by observing response of a biological substance with respect to a physiologically active substance. The method comprises the steps of: providing a mixture solution of the biological substance and a liquid medium containing a solidifying agent to a substrate for thin film formation (S1); solidifying the mixture solution and forming a biological substance-fixed solid thin film (S2); supplying the physiologically active substance to the solid thin film to diffuse the physiologically active substance into the solid thin film (S3); and determining minimum inhibitory concentration (MIC) of the physiologically active substance based on a result obtained by imaging individual response of single cells of the biological substance with respect to the physiologically active substance. [Reference numerals] (AA) Start; (BB) End; (S1) Provide a mixture solution of a biological substance and a liquid medium containing a solidifying agent to a substrate; (S2) Form a biological substance-fixed solid thin film by solidifying the mixture solution; (S3) Diffuse a physiologically active substance into the solid thin film; (S4) Observe the reaction of the biological substance with respect to the physiologically active substance
고형화제를 함유한 액상 매질과 생물학적 물질의 혼합 용액을 박막형성용 구조물에 제공하는 단계; 상기 혼합 용액을 고화시켜 상기 생물학적 물질이 고정된 고체 박막을 형성하는 단계; 생리활성물질을 상기 고체 박막에 공급하여 상기 생리활성물질이 상기 고체 박막 내부로 확산되도록 하는 단계; 및 상기 생리활성물질에 대한 상기 생물학적 물질의 반응을 관찰하는 단계를 포함하는 검사 방법이 제공된다.

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    KR-101770610-B1August 24, 2017주식회사 퀀타매트릭스고정화제를 이용한 단일 세포 추적을 위한 신규한 생리 활성 검사용 구조물
    WO-2014178692-A1November 06, 2014주식회사 퀀타매트릭스, 서울대학교산학협력단Équipement d'essai de culture cellulaire à base de multiples puits microfluidiques